Anti-CSRP2BP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H8E8

Core Product Specifications and Parameters

Biological Background: CSRP2BP Function and Localization

• CSRP2BP, also known as KAT14, ATAC2, or CRP2BP, is a component of the ATAC (ADA2A-containing) complex, a transcriptional coactivator complex with histone acetyltransferase (HAT) activity. It functions as a scaffold to maintain ATAC complex stability and possesses weak intrinsic HAT activity toward histone H4.
• The protein is involved in the regulation of gene expression through chromatin remodeling, specifically acetylating histones H3 and H4. This modification is critical for transcriptional regulation and cellular processes like cell cycle progression.
• Gene family: CSRP2BP belongs to the lysine acetyltransferase family (KAT14). The gene undergoes alternative splicing, producing multiple isoforms. A reference proteome has been identified.
• Subcellular localization: CSRP2BP is found in both the nucleus and the cytoplasm, consistent with its role in chromatin modification and potential cytoplasmic functions.
• Post-translational modifications: CSRP2BP is subject to acetylation and phosphorylation, as indicated by proteomics identification studies. These modifications may regulate its activity and interactions.
• Tissue specificity: Widely expressed in various tissues including skeletal muscle, heart, lung, placenta, brain, liver, pancreas, and kidney. Highest expression is observed in skeletal muscle and heart, with lower levels in lung.
• Required for normal progression through G1 and G2/M phases of the cell cycle, indicating a role in cell growth and division. May be relevant in muscle and heart physiology due to high expression.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 523-782 of human CSRP2BP, a region that may be conserved across isoforms. Consider this when designing experiments to target specific splice variants.
• For Western blot (WB), a band around 89 kDa is expected for the full-length protein, though alternative splicing may produce additional bands. Validate in the relevant sample system.
• For immunofluorescence (IF/ICC), CSRP2BP localizes to the nucleus and cytoplasm; verify signal distribution in your cell type of interest.
• For ELISA, consider using recombinant CSRP2BP protein as a positive control to assess binding specificity.
• Cross-reactivity with rat is indicated; ensure appropriate controls when working with rat samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with comprehensive access to high-quality IVD raw materials. For targets like CSRP2BP involved in chromatin biology and epigenetic regulation, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities cover rare targets and support your entire development process—from concept to clinic.