Anti-CSRP1 Polyclonal Antibody for WB, IP, ELISA, RIP - P21291

Core Product Specifications and Parameters

Biological Background: CSRP1 Function and Localization

• CSRP1, also known as cysteine and glycine-rich protein 1 (CRP1), is a member of the cysteine-rich protein family, characterized by two LIM zinc-binding domains.
• The protein functions as a dual zinc-finger transcription factor, suggesting involvement in regulation of gene expression during development.
• According to UniProt records, CSRP1 could play a role in neuronal development, indicating its importance in the central nervous system.
• Subcellular localization studies place CSRP1 predominantly in the nucleus, consistent with its putative role in transcriptional control.
• Multiple post-translational modifications have been identified, including acetylation, phosphorylation, and ubiquitination, which may modulate its activity or stability.
• The presence of isopeptide bonds and Ubl conjugation further implies complex regulatory mechanisms governing CSRP1 function.
• CSRP1 is classified under the LIM domain repeat family, with structural motifs that mediate protein-protein interactions and metal-ion coordination.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody comprises the full-length human CSRP1 protein (amino acids 1–193), which may provide robust detection of both native and denatured forms.
• For Western blotting, a specific band around 21 kDa is expected; we recommend running appropriate molecular weight markers and positive controls to confirm results.
• In immunoprecipitation experiments, verify antibody specificity by including isotype controls or pre-immune serum, and consider the impact of potential post-translational modifications on precipitation efficiency.
• For ELISA applications, perform checkerboard titrations to determine optimal coating antigen and antibody dilutions; the assay may be used to quantify CSRP1 in cell or tissue lysates.
• When using this antibody for RNA immunoprecipitation (RIP), include RNase-free conditions and appropriate cross-linking controls, as CSRP1’s nuclear localization supports its involvement in RNA-protein complexes.
• Given the reported cross-reactivity with mouse, validate the antibody in your specific mouse model system and tissue type, as expression levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CSRP1-related studies, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your immunoassay development. Whether you need rare target raw-material sourcing or production-scale quantities, our B2B platform accelerates your diagnostic pipeline with reliable, customizable supply solutions.