Anti-CSNK2A2 Rabbit Polyclonal Antibody for WB and ELISA - P19784

Core Product Specifications and Parameters

Biological Background: CSNK2A2 Function and Localization

• Catalytic subunit of the constitutively active serine/threonine-protein kinase CK2, which phosphorylates a broad range of substrates containing acidic residues C-terminal to the target serine or threonine. Related references: PMID:11239457, PMID:11704824, PMID:16193064
• Regulates diverse cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection. Related references: PMID:11704824, PMID:16193064, PMID:30898438
• Acts as a signaling hub that integrates multiple pathways; functions in the p53-dependent spindle assembly checkpoint to maintain G2 arrest upon spindle damage and is required for p53-mediated apoptosis. Related references: PMID:12631575, PMID:19387551, PMID:19387552, PMID:11239457
• Phosphorylates key DNA repair proteins such as MDC1, RAD9A, RAD51, and HTATSF1, promoting their recruitment to DNA damage sites. Related references: PMID:20545769, PMID:21482717, PMID:22325354
• Directly phosphorylates RNA polymerases I, II, III, and IV, as well as numerous transcription factors (e.g., NF-kappa-B, STAT1, MYC, JUN), thereby regulating transcription globally. Related references: PMID:12631575, PMID:19387551, PMID:19387552
• Localizes to both the nucleus and cytoplasm, and also functions as an ectokinase that phosphorylates extracellular proteins. The protein is subject to acetylation and phosphorylation, which may modulate its activity. Related references: PMID:12631575, PMID:19387551, PMID:19387552

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions and loading 20–30 µg of total cell lysate. Test a range of antibody dilutions (e.g., 1:500–1:2000) to determine the optimal signal-to-noise ratio, and include appropriate positive/negative controls (e.g., knockdown or overexpression lysates).
• The recombinant immunogen spanning amino acids 1–350 may enhance recognition of multiple epitopes; however, polyclonal antibodies can exhibit lot-to-lot variability. Validate each new batch in the relevant sample system.
• In ELISA, the antibody may perform well as a detection reagent when paired with a suitable capture antibody. Titrate the coating antigen and detection antibody to optimize assay sensitivity and specificity.
• The reported mouse cross-reactivity permits use in mouse models, but confirm reactivity in your specific tissue or cell type before running large experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, specializing in validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target procurement. This anti-CSNK2A2 antibody supports research into kinase signaling, cell cycle regulation, and cancer biology, and we are equipped to assist with custom development and integrated supply chain solutions tailored to your diagnostic or research needs.