Anti-CSNK2A2 Polyclonal Antibody for WB, IF/ICC, ELISA - P19784

Core Product Specifications and Parameters

Biological Background: CSNK2A2 Function and Localization

• CSNK2A2 is the catalytic alpha' subunit of casein kinase II, a constitutively active serine/threonine kinase that phosphorylates numerous substrates with acidic residues downstream of the phosphoacceptor site, regulating fundamental cellular processes. Related references: PMID:11239457 PMID:11704824 PMID:16193064
• It acts as a regulatory nexus, integrating signals to control cell cycle progression, apoptosis, transcription, and viral infection; it participates in the p53/TP53-dependent spindle assembly checkpoint, maintaining G2 arrest upon spindle damage. Related references: PMID:12631575 PMID:19387551 PMID:19387552 PMID:30898438
• CSNK2A2 mediates p53/TP53-dependent apoptosis by phosphorylating p53 at Ser-392 after UV irradiation, and also phosphorylates caspases CASP9 and CASP2 to negatively regulate apoptosis. Related references: PMID:11239457 PMID:12631575 PMID:19387551 PMID:19387552
• The kinase phosphorylates DNA repair proteins, including MDC1, RAD9A, RAD51, and HTATSF1, facilitating their recruitment to sites of DNA damage. Related references: PMID:20545769 PMID:21482717 PMID:22325354
• It regulates transcription by directly phosphorylating RNA polymerases I, II, III, and IV, and numerous transcription factors such as NF-kappa-B, STAT1, CREB1, MYC, and JUN. Related references: PMID:12631575 PMID:19387551 PMID:19387552
• CSNK2A2 is involved in chaperone function through phosphorylation of Hsp90 and co-chaperones FKBP4 and CDC37, and modulates Wnt signaling by phosphorylating CTNNB1 and LEF1. Related references: PMID:19387549 PMID:19387550
• The protein localizes to both the nucleus and cytoplasm and is subject to acetylation and extensive phosphorylation.
• It exerts ectokinase activity on extracellular substrates, plays a role in the life cycle of several viruses (EBV, HSV, HBV, HCV, HIV, CMV, HPV), and may phosphorylate histone H2A at Ser-1. Related references: PMID:12631575 PMID:19387551 PMID:19387552 PMID:38334665

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250–350 of human CSNK2A2; the antibody is expected to recognize the full-length ~41 kDa protein.
• For western blot, a band around 41 kDa should be observed in lysates from human, mouse, and rat tissues; consider including appropriate positive and negative controls.
• In IF/ICC, optimize fixation and permeabilization conditions, as CSNK2A2 localizes to both nucleus and cytoplasm.
• For ELISA, use recombinant protein or the immunizing peptide as a standard; validate the assay in your specific sample matrix.
• Due to high sequence similarity with CSNK2A1, potential cross-reactivity may occur; confirm specificity via siRNA knockdown or isoform-specific inhibitors.

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