Anti-CSNK1G1 Rabbit Polyclonal Antibody for WB and ELISA - Q9HCP0

Core Product Specifications and Parameters

Biological Background: CSNK1G1 Function and Localization

• CSNK1G1 encodes casein kinase I isoform gamma-1, a serine/threonine-protein kinase that preferentially phosphorylates acidic substrates such as caseins.
• This kinase is a key component of the Wnt signaling pathway, where it participates in the phosphorylation of downstream effectors to regulate signal transduction.
• CSNK1G1 localizes predominantly to the cytoplasm, consistent with its role in intracellular signaling cascades.
• The protein is characterized as a phosphoprotein and contains an ATP-binding domain essential for its kinase activity (keywords: ATP-binding, Nucleotide-binding).
• It is known to phosphorylate the checkpoint protein CLSPN, implicating it in cell cycle regulation and DNA damage response pathways.
• Alternative splicing of the CSNK1G1 gene generates multiple isoforms, expanding the functional diversity of this kinase.
• CSNK1G1 is detected in proteomics studies and is classified under reference proteome, indicating its broad expression and fundamental cellular role.
• Additionally, it regulates fast synaptic transmission mediated by glutamate, highlighting a specialized function in neuronal signaling (by similarity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 298-422 of human CSNK1G1 (NP_071331.2), targeting a region within the C-terminal portion of the protein. Verify sequence homology if used in non-human species.
• For Western blot, the predicted molecular weight is approximately 49 kDa. Include appropriate positive controls (e.g., HeLa or HEK293 lysates) and validate with a loading control.
• For ELISA, optimize coating antigen and primary antibody concentration. Consider using the recombinant immunogen as a positive control to establish assay sensitivity.
• As a polyclonal antibody, batch-to-batch consistency should be confirmed if used in quantitative or regulated assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, ensuring a seamless supply chain from concept to clinic. In the area of Wnt signaling and kinase research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-CSNK1G1, along with bulk ancillary reagents. For rare or emerging targets, our global network enables rapid procurement, helping diagnostic developers accelerate assay development.