Anti-CSNK1E Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49674

Core Product Specifications and Parameters

Biological Background: CSNK1E Function and Localization

• CSNK1E encodes casein kinase I isoform epsilon (CKIε), a serine/threonine-protein kinase with aliases CKIe, HCKIE, and CKIepsilon.
• It preferentially phosphorylates acidic proteins like caseins, defining its casein kinase activity.
• CSNK1E participates in Wnt signaling by phosphorylating DVL1 and DVL2, facilitating signal transduction. Related references: PMID:12556519 PMID:23413191
• It is a central component of the circadian clock, regulating the phosphorylation and rhythmicity of PER1 and PER2 to determine circadian period length. Related references: PMID:16790549 PMID:15917222
• CSNK1E localizes to both the cytoplasm and nucleus, consistent with its roles in signal transduction and gene regulation.
• It is broadly expressed across tissues, including brain, heart, lung, liver, pancreas, kidney, placenta, and skeletal muscle, and in monocytes and lymphocytes but not granulocytes.
• Post-translational modifications such as phosphorylation and methylation regulate its activity and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 247–416 within the kinase domain, potentially recognizing major isoforms; validate specificity in your target species and sample type.
• As a polyclonal antibody, lot-to-lot consistency should be assessed by performing a pilot experiment; consider using a positive control lysate known to express CSNK1E.
• For western blot, a band near 47 kDa is expected; however, post-translational modifications or splice variants may result in additional bands.
• In IHC-P and IF/ICC, optimize antigen retrieval and permeabilization conditions, and include appropriate controls to confirm staining specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with premium IVD raw materials. This anti-CSNK1E antibody supports circadian rhythm and Wnt signaling studies, and we can further assist with validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, and the sourcing of rare targets to streamline your assay development pipeline.