Anti-CSMD3 Polyclonal Antibody for WB, ELISA - Q7Z407

Core Product Specifications and Parameters

Biological Background: CSMD3 Function and Localization

• CUB and sushi domain-containing protein 3 (CSMD3) is a large transmembrane protein of approximately 406 kDa, also known as CUB and sushi multiple domains protein 3.
• CSMD3 is a member of the CSMD family, characterized by multiple CUB (Complement C1r/C1s, Uegf, Bmp1) and sushi (CCP/SCR) domains, which are involved in protein-protein interactions and cell adhesion.
• The primary function of CSMD3 is involvement in dendrite development, contributing to neuronal morphology and synaptic connectivity.
• CSMD3 is localized at the cell membrane, with its extracellular region containing the CUB and sushi domains potentially mediating interactions with other cell surface or extracellular matrix components.
• Tissue expression is widespread but low, with relatively higher levels in the brain (including cerebellum, substantia nigra, thalamus, hippocampus, fetal brain, and spinal cord) and testis, supporting a role in neural tissues.
• Post-translational modifications include glycosylation and disulfide bond formation, typical for cell surface proteins, which may be important for structural stability and function.
• Multiple isoforms are generated through alternative splicing, which may lead to differential domain compositions and functional diversification.
• The protein is proteomically identified and serves as a reference proteome component, underlining its biological significance.

Experimental Guidance and Technical Tips

• Based on the immunogen design (aa 220-440 of human CSMD3), the antibody may recognize multiple isoforms unless the epitope is within a conserved region. Validate the expected band size (~406 kDa) in WB with appropriate molecular weight markers and prolonged transfer time for high-molecular-weight proteins.
• For WB, consider using brain or testis lysates as positive controls, given the higher expression of CSMD3 in these tissues. Ensure complete denaturation and reducing conditions to resolve the large transmembrane protein.
• For ELISA, the antibody can be used for detection of recombinant CSMD3 fragments or endogenous protein in lysates. Optimization of coating and blocking conditions is recommended to account for steric hindrance due to protein size.
• Due to the transmembrane nature, native protein may be embedded in membranes; consider using detergent-containing buffers for solubilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers as a one-stop IVD raw-material sourcing bridge, offering a comprehensive portfolio that includes validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. For targets like CSMD3, which play critical roles in neurodevelopment, CamelBio can supply optimized antibody pairs and support the development of reliable immunoassays. From rare target sourcing to bulk procurement, our integrated supply chain accelerates your journey from concept to clinic.