Anti-CSK Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P41240

Core Product Specifications and Parameters

Biological Background: CSK Function and Localization

• CSK is a non-receptor tyrosine-protein kinase that plays a critical role in regulating cell growth, differentiation, migration, and immune response.
• It phosphorylates the C-terminal tyrosine residues of Src-family kinases (SFKs) such as LCK, SRC, HCK, FYN, LYN, and YES1, promoting an inactive conformation through intramolecular interactions.
• CSK suppresses signaling from various surface receptors including T-cell receptor (TCR) and B-cell receptor (BCR) by maintaining SFKs in an inactive state.
• It may also act as a negative regulator of EGFR and STAT3 signaling pathways (PubMed:26918609). Related references: PMID:26918609
• Subcellularly, CSK localizes to the cytoplasm and is recruited to the cell membrane via interactions with transmembrane or adapter proteins for SFK inhibition.
• Tissue expression is detected in lung and macrophages, consistent with its role in immune regulation.
• The protein contains SH2 and SH3 domains and requires ATP, magnesium, or manganese for kinase activity; it is subject to acetylation and phosphorylation modifications.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 188–450, which includes the catalytic kinase domain and part of the C-terminal region. This design may enable recognition of both full-length CSK and potentially truncated or cleaved forms.
• For Western blot (WB), a band at approximately 51 kDa is expected; consider using reducing conditions and a suitable positive control (e.g., Jurkat or A549 cell lysates) for optimal detection.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization protocols may need optimization; cytoplasmic and membrane staining patterns are anticipated.
• For ELISA applications, this polyclonal antibody may be used as a capture or detection reagent; perform titration and cross-reactivity tests with the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-CSK polyclonal antibody as part of its comprehensive catalog of IVD raw materials for immunodiagnostics and research. With expertise in antibody development, CamelBio provides validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to support your CSK-focused studies. For custom sourcing of rare targets, our team ensures reliable supply and technical consultation from concept to clinic.