Anti-CSK Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P41240

Core Product Specifications and Parameters

Biological Background: CSK Function and Localization

• CSK (Tyrosine-protein kinase CSK, C-Src kinase) is a non-receptor tyrosine-protein kinase that serves as a master negative regulator of Src-family kinases (SFKs).
• CSK phosphorylates conserved tyrosine residues in the C-terminal tails of SFKs (e.g., SRC, LCK, FYN), inducing an intramolecular interaction that locks them in an inactive conformation and suppresses downstream signaling.
• By inhibiting SFKs, CSK modulates signaling from T-cell and B-cell receptors, and negatively regulates EGFR and STAT3 pathways. Related references: PMID:26918609
• CSK is primarily cytoplasmic and is recruited to the plasma membrane via binding to transmembrane or adapter proteins to access its membrane-associated substrates.
• The protein is expressed in lung tissue and macrophages, aligning with its role in immune regulation.
• CSK features SH2 and SH3 domains and an ATP-binding kinase domain, and is subject to acetylation and phosphorylation modifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human CSK, which may favor detection of the full-length protein across species.
• For Western blot, prepare lysates from physiologically relevant samples (e.g., lung, immune cells) and consider using a standard SDS-PAGE protocol; CSK has a predicted molecular weight of ~51 kDa.
• For immunohistochemistry (paraffin sections), validate antigen retrieval conditions and antibody dilution; CSK cytoplasmic and membrane localization should be assessed.
• The antibody shows cross-reactivity with mouse and rat CSK, suggesting the epitope is conserved; however, verify species-specific performance if switching models.
• In ELISA, use appropriate capture/detection formats and confirm linearity with recombinant CSK protein if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research laboratories in acquiring premium raw materials for immunoassay development. This anti-CSK antibody fits into signal transduction and immune-oncology reagent panels, and we can also supply validated antibody pairs, ancillary reagents, and bulk quantities. For challenging or niche targets like CSK, CamelBio leverages its technical network to deliver reliable sourcing solutions—helping you move from concept to clinic efficiently.