Anti-CSK (C-Src kinase) Rabbit Polyclonal Antibody for WB, ELISA - P41240

Core Product Specifications and Parameters

Biological Background: CSK Function and Localization

• CSK (C-Src kinase) is a non-receptor tyrosine-protein kinase that plays critical roles in regulating cell growth, differentiation, migration, and immune responses.
• It phosphorylates tyrosine residues in the C-terminal tails of Src-family kinases (SFKs) such as LCK, SRC, HCK, FYN, LYN, and YES1, promoting an inactive conformation and thereby inhibiting their activity.
• CSK is recruited to the plasma membrane via interactions with transmembrane or adaptor proteins to effectively inhibit SFKs and suppress signaling from receptors like T-cell receptor (TCR) and B-cell receptor (BCR).
• It may function as a negative regulator of the EGFR and STAT3 signaling pathways, as supported by experimental evidence (PMID:26918609). Related references: PMID:26918609
• The protein contains SH2 and SH3 domains and belongs to the tyrosine-protein kinase family, requiring ATP and metal ions such as magnesium or manganese for catalytic activity.
• Subcellularly, CSK localizes to the cytoplasm and cell membrane, consistent with its membrane-proximal signaling roles.
• It is predominantly expressed in lung and macrophages, suggesting tissue-specific regulatory functions.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein corresponding to amino acids 1-200 of human CSK, which covers a significant portion of the SH3 and SH2 domains, providing broad epitope recognition.
• For Western blotting, a band around 51 kDa is expected; validate using positive control lysates from human, mouse, or rat tissues (e.g., lung or macrophage cell lines) due to known cross-reactivity.
• To detect CSK activity or localization changes, consider stimulating or inhibiting upstream signaling pathways (e.g., EGFR or TCR) as CSK dynamically associates with the plasma membrane.
• For ELISA applications, titrate the antibody against the recombinant immunogen or native protein to establish optimal working concentrations; include appropriate negative controls to assess background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents to support your development from concept to clinic. This anti-CSK antibody is ideal for researchers studying Src-family kinase regulation, immune signaling, and cancer biology. Beyond this product, CamelBio can assist in sourcing validated antibody pairs, bulk reagents, and rare-target materials to accelerate your assay development and manufacturing scale-up.