Anti-CSF3R Polyclonal Antibody for WB, ELISA - Q99062

Core Product Specifications and Parameters

Biological Background: CSF3R Function and Localization

• CSF3R encodes the receptor for granulocyte colony-stimulating factor (CSF3), a critical regulator of granulocytic maturation.
• The receptor is essential for the proliferation, differentiation, and survival of cells along the neutrophilic lineage.
• CSF3R may also participate in adhesion or recognition events at the cell surface, suggesting additional roles beyond cytokine signaling.
• Multiple isoforms exist due to alternative splicing; subcellular localization includes cell membrane and secreted forms.
• Isoform GCSFR-2 is specifically found in leukemia U-937 cells, while isoform GCSFR-3 is highly expressed in placenta.
• Tissue expression includes myelogenous leukemia cell line KG-1, bone marrow cells, peripheral blood granulocytes, and placenta.
• The protein is characterized by an extracellular region with immunoglobulin-like domains, a single transmembrane helix, and conserved disulfide bonds.
• Disease-associated variants have been reported, and the protein is subject to glycosylation and proteolytic processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (aa 650-750), which may influence detection of certain isoforms or processed forms; verify reactivity in the relevant sample system.
• For Western blot, consider loading positive control lysates from CSF3R-expressing cell lines (e.g., KG-1, U-937) or peripheral blood granulocytes and check for a band near 92 kDa.
• In ELISA applications, the peptide immunogen can serve as a positive coating antigen, but native CSF3R recognition should be validated in the intended biological matrix.
• Include appropriate negative controls (e.g., knockout or knockdown lysates) to confirm band specificity in Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, technical services, and consulting. This high-quality antibody supports granulocyte biology and leukemia research, and we can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Let CamelBio streamline your development pipeline—from concept to clinic.