Anti-CSF3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P09919

Core Product Specifications and Parameters

Biological Background: CSF3 Function and Localization

• Granulocyte colony-stimulating factor (GCSF), also known as pluripoietin, is encoded by the CSF3 gene (GeneID: 1440) on chromosome 17.
• It belongs to the granulocyte/macrophage colony-stimulating factor family, cytokines that regulate production, differentiation, and function of granulocytes and monocytes-macrophages.
• CSF3 specifically induces the proliferation and differentiation of granulocyte precursor cells into mature neutrophils, playing a central role in innate immunity.
• The protein is secreted and acts through specific cell surface receptors (CSF3R) on hematopoietic cells to trigger intracellular signaling cascades.
• UniProt keywords highlight key features: alternative splicing generates isoforms, disulfide bonds stabilize the tertiary structure, and N-linked glycosylation influences activity and half-life.
• Post-translational modifications include signal peptide cleavage for secretion and glycosylation at specific asparagine residues.
• While no strict tissue specificity is reported, CSF3 is predominantly produced by immune cells, endothelial cells, and fibroblasts in response to pro-inflammatory stimuli.
• Recombinant forms of CSF3 are used clinically (e.g., filgrastim) to treat chemotherapy-induced neutropenia, underscoring its pharmaceutical importance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30-207 of the mature human CSF3 sequence, likely covering the receptor-binding domain, and may detect both endogenous and recombinant protein.
• For Western blot, the predicted molecular weight is 22 kDa; however, glycosylation can shift the apparent mass. Use reducing SDS-PAGE and consider a positive control like recombinant human GCSF.
• In IHC-P, antigen retrieval (heat or enzymatic) may be required; validate with bone marrow or inflamed tissue sections where granulopoiesis is active.
• For ELISA, a matched detection antibody may improve sensitivity; check cross-reactivity against mouse and rat CSF3 if working across species.
• As a polyclonal antibody, lot-to-lot variability is possible; maintain consistent lot numbers and include a standard reference control in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement by offering validated antibodies, recombinant proteins, and ancillary reagents under one roof. This anti-CSF3 polyclonal antibody supports granulocyte colony-stimulating factor research in hematopoiesis, immunology, and translational oncology. From bulk monoclonal antibodies to rare target sourcing, CamelBio backs your diagnostic development from concept to clinic.