Anti-CSF2RB Rabbit Polyclonal Antibody for WB, ELISA - P32927

Core Product Specifications and Parameters

Biological Background: CSF2RB Function and Localization

• CSF2RB encodes the cytokine receptor common subunit beta (also known as CD131 or CDw131), which is the shared signal-transducing subunit for the interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors.
• Upon ligand binding, CSF2RB forms a heterodimeric complex with specific alpha subunits (IL3RA, IL5RA, or CSF2RA), initiating intracellular signaling cascades.
  Related references: PMID:1495999
• CSF2RB constitutively associates with JAK1; ligand-induced receptor activation leads to JAK1 stimulation and subsequent phosphorylation of STAT transcription factors, propagating the JAK-STAT signal transduction pathway.
  Related references: PMID:9516124
• The receptor is critical for the proliferation, differentiation, and survival of hematopoietic progenitor cells, playing a pivotal role in innate and adaptive immunity.
• Subcellularly, CSF2RB is a transmembrane protein localized to the plasma membrane (as supported by UniProt annotation).
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation, contributing to receptor stability and signaling regulation. Multiple isoforms are produced by alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an extracellular region (aa 200-443) of human CSF2RB, which is expected to recognize both native and denatured protein forms, making it suitable for western blotting under reducing conditions.
• For ELISA applications, consider using a matched pair approach with a detection antibody targeting a distal epitope to avoid competition; perform checkerboard titrations to optimize sensitivity.
• Although cross-reactivity with mouse is reported, we recommend validating specificity in the relevant sample panels, as polyclonal antibodies may exhibit batch-dependent background.
• Use appropriate positive and negative controls (e.g., cell lines with known CSF2RB expression) and optimize blocking conditions to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined pathway for cytokine receptor-focused IVD development by providing validated antibody pairs, optimized polyclonal antibodies such as anti-CSF2RB, and bulk ancillary reagents. Our integrated platform simplifies procurement of rare-target raw materials, supporting your projects from initial research to clinical application with robust supply continuity and technical consultation.