Anti-CSF2RA Rabbit Polyclonal Antibody for IF/ICC, ELISA - P15509

Core Product Specifications and Parameters

Biological Background: CSF2RA Function and Localization

• Encodes the granulocyte-macrophage colony-stimulating factor receptor subunit alpha (CSF2RA), also designated CDw116.
• Functions as a low-affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF), transducing signals critical for hematopoietic cell proliferation, differentiation, and functional activation.
• The gene CSF2RA (also known as CSF2R, CSF2RY) generates multiple isoforms via alternative splicing.
• Localizes primarily to the cell membrane as a transmembrane receptor; secreted isoforms are also produced.
• Belongs to the type I cytokine receptor family and is a key component of the GM-CSF receptor complex.
• Contains a signal peptide, an extracellular domain with disulfide bonds, a single transmembrane helix, and is post-translationally modified by glycosylation.
• Genetic variations in CSF2RA are associated with disease phenotypes (e.g., pulmonary alveolar proteinosis), as noted in UniProt keywords.
• The protein is expressed on the surface of hematopoietic cells and plays a role in innate immunity and inflammation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 23–150), suggesting the antibody likely recognizes the receptor's N-terminal region, potentially detecting both membrane-bound and secreted isoforms.
• As a rabbit polyclonal antibody, it may exhibit lot-to-lot variability; users are advised to validate performance in their specific assay conditions.
• For IF/ICC, proper fixation and permeabilization protocols should be optimized for the target cell type, as membrane localization may require gentle permeabilization.
• For ELISA, pairing with a detection antibody that binds a distinct epitope is recommended; consider using recombinant GM-CSF as a capture ligand to confirm specificity.
• If considering western blotting, note the predicted molecular weight of ~46 kDa; denaturing and reducing conditions may affect detection of the glycosylated protein.
• The antibody shows cross-reactivity with mouse, making it suitable for studies in both human and mouse models; however, cross-reactivity with other species should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-CSF2RA antibody, to accelerate your diagnostic development in hematology and immunology. Beyond antibodies, we provide bulk ancillary reagents and can source rare-target raw materials to support your entire product pipeline from concept to clinic. Partner with CamelBio for a seamless one-stop sourcing experience.