Anti-CSF2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P04141

Core Product Specifications and Parameters

Biological Background: CSF2 Function and Localization

• CSF2, also known as Granulocyte-macrophage colony-stimulating factor (GM-CSF), is a secreted cytokine with aliases including CSF, Molgramostin, and Sargramostim.
• It functions as a growth factor that stimulates the proliferation and differentiation of hematopoietic precursor cells into granulocytes, macrophages, eosinophils, and erythrocytes.
• CSF2 is a member of the colony-stimulating factor family and plays a crucial role in innate and adaptive immunity.
• The protein is a glycoprotein containing disulfide bonds, essential for its structural integrity and receptor binding.
• Subcellular localization is predominantly extracellular, being secreted from activated T cells, macrophages, and other cell types.
• CSF2 is used pharmaceutically (e.g., Molgramostin, Sargramostim) to support bone marrow recovery after chemotherapy.
• The mature protein has a molecular weight of approximately 16 kDa (predicted from sequence: 16295 Da), consistent with the measured weight of 16 kDa in Western blot under reducing conditions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100) of human CSF2, suggesting recognition of native and possibly reduced protein forms.
• For Western blot, a band at ~16 kDa is expected. Use reducing SDS-PAGE and validate with a positive control such as stimulated human PBMCs or recombinant CSF2.
• In immunofluorescence, optimize fixation/permeabilization; a secretion inhibitor (e.g., Brefeldin A) may be needed for intracellular staining.
• For ELISA, the antibody is suitable as capture or detection reagent; pairing with a clone against a distinct epitope is advised for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target custom development. This anti-CSF2 antibody fits into immunoassay and cytokine research applications, and our team can support your project with complementary materials and technical consultation from concept to clinic.