Anti-CSF1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P09603

Core Product Specifications and Parameters

Biological Background: CSF1 Function and Localization

• Macrophage colony-stimulating factor 1 (CSF1), also designated lanimostim or PG-M-CSF, is a secreted cytokine and transmembrane protein that governs the survival, proliferation, and differentiation of hematopoietic progenitor cells, particularly mononuclear phagocytes.
• CSF1 drives the release of pro-inflammatory chemokines, positioning it as a key modulator of innate immunity and inflammatory responses.
• The cytokine is essential for osteoclastogenesis and bone resorption; it is indispensable for normal skeletal development and maintenance.
• Beyond immunity and bone, CSF1 supports both male and female fertility and facilitates cell adhesion, migration, and actin-driven membrane ruffle formation.
• CSF1 contributes to lipoprotein clearance, highlighting metabolic cross-talk with immune function.
• At the subcellular level, CSF1 localizes to the cell membrane and is shed into the extracellular space, consistent with dual autocrine and paracrine signaling roles.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation, while alternative splicing generates multiple isoforms, including a proteoglycan form.

Experimental Guidance and Technical Tips

• The recombinant immunogen spanning amino acids 33–190 of the mature CSF1 sequence (NP_757351.1) covers the receptor-binding region; when setting up western blot, consider that the full-length precursor (~60 kDa) and potentially cleaved or glycosylated variants may appear. Validate detection in your sample matrix.
• For IF/ICC, fixation and permeabilization protocols should be optimized to preserve both the secreted and membrane-bound CSF1 pools; inclusion of a secretion inhibitor may enhance intracellular signal.
• In ELISA applications, this polyclonal reagent can serve as a capture or detection antibody; however, pairing with a suitable monoclonal antibody is recommended to achieve desired specificity and dynamic range.
• Cross‑reactivity is specified for human CSF1; if testing other species, perform sequence alignment of the immunogen region and validate recognition empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development for CSF1-related assays by providing a comprehensive portfolio of raw materials, including this polyclonal antibody alongside optimized recombinant proteins and complementary monoclonal reagents. Whether you need diagnostic‑grade antibody pairs, ancillary reagents in bulk, or sourcing of rare‑target materials, CamelBio bridges your workflow from concept to clinic with expert technical consultation.