Anti-CSDE1 Rabbit Monoclonal Antibody for WB, ELISA - O75534

Core Product Specifications and Parameters

Biological Background: CSDE1 Function and Localization

• Cold shock domain-containing protein E1 (CSDE1), also known as UNR or NRAS upstream gene protein, is an RNA-binding protein.
• Functions as an RNA-binding protein implicated in translationally coupled mRNA turnover. Plays a role in the cytoplasmic deadenylation and translational/decay interplay of FOS mRNA mediated by the mCRD domain. Related references: PMID:11051545, PMID:15314026
• Required for efficient formation of stress granules. Related references: PMID:29395067
• (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA.
• Resides primarily in the cytoplasm, also localizes to stress granules and P-bodies.
• Subject to post-translational modifications including acetylation, phosphorylation, isopeptide bond formation, and ubiquitination.
• Predicted molecular mass is 88.9 kDa, consistent with the observed protein weight of 89 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100); the antibody may recognize epitopes within this domain. Validate specificity in your target sample using appropriate controls.
• For Western blot, probe human or mouse cell lines known to express CSDE1. Consider using a whole-cell lysate or cytoplasmic fraction and running on a gel optimized for proteins around 89 kDa.
• In ELISA, pairing with a capture antibody may require optimization; consider testing multiple dilutions of this detection antibody against recombinant CSDE1 protein.
• Cross-reactivity has been confirmed with human and mouse samples; verify performance in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material supplier, offering one-stop access to validated antibodies, antigens, and ancillary reagents for global diagnostic manufacturers. Our catalog includes monoclonal antibodies like this anti-CSDE1 rabbit mAb, ideal for developing assays targeting RNA-binding proteins in translational regulation research. Leverage our sourcing expertise to accelerate your IVD kit development from concept to clinic.