Anti-CS Polyclonal Antibody for WB, IF/ICC, ELISA - O75390

Core Product Specifications and Parameters

Biological Background: CS Function and Localization

• Citrate synthase (CS) is a mitochondrial enzyme encoded by the CS gene, also referred to as citrate (Si)-synthase.
• It catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, the initial and rate-limiting step of the tricarboxylic acid (TCA) cycle.
• The enzyme resides in the mitochondrial matrix, where it plays a central role in aerobic energy production and carbohydrate metabolism.
• Post-translational modifications such as acetylation, methylation, and ubiquitination have been reported, potentially modulating enzyme activity or stability.
• CS possesses a mitochondrial transit peptide that directs its import into mitochondria.
• The mature protein has a molecular weight of approximately 52 kDa.
• As a key metabolic regulator, CS is vital for cellular respiration and has been studied in the context of mitochondrial dysfunction and cancer metabolism.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans amino acids 167–466 of human CS, covering a large portion of the protein; therefore, the polyclonal antibody is likely to recognize multiple epitopes.
• For Western blotting, a single band at ~52 kDa should be detected in samples from human, mouse, and rat, consistent with the predicted molecular weight.
• In immunofluorescence and immunocytochemistry, a mitochondrial staining pattern is expected, reflecting the enzyme's subcellular localization.
• Since the antibody is polyclonal, it may offer increased sensitivity and robustness across species due to epitope diversity, but individual lot validation is recommended.
• Optimize sample preparation (e.g., reducing conditions for WB, fixation method for IF) to achieve the best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-CS polyclonal antibody supports research into mitochondrial metabolism and the TCA cycle. With a broad portfolio of validated antibodies, bulk ancillary reagents, and rare target sourcing capabilities, CamelBio streamlines the procurement of critical raw materials from concept to clinic.