Anti-CRYGC Polyclonal Antibody for WB, IF/ICC, ELISA - P07315

Core Product Specifications and Parameters

Biological Background: CRYGC Function and Localization

• CRYGC, also known as gamma-crystallin C, gamma-C-crystallin, gamma-crystallin 2-1, and gamma-crystallin 3, is a member of the beta/gamma-crystallin family.
• It functions as a dominant structural protein of the vertebrate eye lens, contributing to lens transparency and refractive index.
• Crystallins are encoded by a gene family that includes several gamma-crystallin genes (CRYGA, CRYGB, etc.), which share high sequence similarity and lens-specific expression.
• Mutations in CRYGC and other crystallins are associated with congenital cataracts, as indicated by the UniProt keywords "Cataract" and "Disease variant."
• The protein has a molecular weight of approximately 21 kDa (calculated from sequence) and is subject to post-translational modifications such as methylation.
• CRYGC is exclusively expressed in the lens, consistent with its role in maintaining lens structure and function.
• It is identified in proteomics studies and forms part of the reference proteome for human.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fusion protein containing amino acids 1-174 of human CRYGC. For Western blot, a typical starting dilution of 1:500-1:2000 may be evaluated with lens tissue lysates or cell lines overexpressing CRYGC.
• In immunofluorescence/immunocytochemistry, consider using a dilution of 1:50-1:200 and optimize fixation and permeabilization conditions for lens cells or tissue sections.
• For ELISA, the antibody can be tested as a capture or detection reagent; validate with recombinant CRYGC protein or lens extracts.
• Cross-reactivity with mouse and rat is reported; however, sequence homology should be checked for specific applications, and validation in the relevant species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to IVD raw materials and technical services, supporting projects from concept to clinic. This anti-CRYGC polyclonal antibody serves as a reliable tool for eye lens research and cataract-related biomarker studies. As your sourcing partner, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare target raw materials—all designed to accelerate your assay development and production workflows.