Anti-CRYBB2 Rabbit Polyclonal Antibody for WB, ELISA - P43320

Core Product Specifications and Parameters

Biological Background: Beta-Crystallin B2 Function and Localization

• CRYBB2 encodes beta-crystallin B2, a major structural protein of the vertebrate eye lens.
• Crystallins are essential for maintaining lens transparency and high refractive index.
• Beta-crystallin B2 belongs to the beta/gamma-crystallin superfamily, characterized by Greek key motifs.
• The protein forms homo- and hetero-oligomeric complexes with other beta-crystallins, contributing to the dense packing of lens fiber cells.
• Mutations in CRYBB2 are linked to cataract formation, underscoring its critical role in lens stability.
• Post-translational modifications such as acetylation may regulate protein interactions and long-term stability.
• As a crystallin, beta-crystallin B2 exhibits extremely low turnover, serving as a lifelong structural component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CRYBB2 protein (residues 1–205), enabling recognition of both native and denatured forms.
• For Western blot analysis, reducing and denaturing conditions are recommended; a band at approximately 23 kDa is expected in lens lysates.
• When using ELISA, coat the antigen appropriately and consider using lens tissue extracts or recombinant protein for standard curves.
• Given the sequence conservation, cross-reactivity with mouse and rat has been demonstrated; validate in other species if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in one-stop sourcing of IVD raw materials, including validated antibodies for ocular disease research. This anti-CRYBB2 polyclonal antibody is available alongside matched polyclonal/monoclonal pairs, bulk ancillary reagents, and customized procurement for rare targets. Whether you need research-grade reagents or scalable diagnostic raw materials, CamelBio streamlines your supply chain from discovery to clinical application.