Anti-CRYBB1 Rabbit Polyclonal Antibody for WB, ELISA - P53674

Core Product Specifications and Parameters

Biological Background: Beta-crystallin B1 Function and Localization

• Beta-crystallin B1 (CRYBB1) is a major structural protein of the vertebrate eye lens, critical for maintaining lens transparency and refractive function.
• Belongs to the beta/gamma-crystallin superfamily, characterized by Greek key motifs; the protein comprises two domains forming a stable globular structure.
• Post-translational modifications, including acetylation, have been identified and may regulate protein stability and interactions.
• Mutations in CRYBB1 are associated with congenital cataract (cataract type 17, CTRCT17), underscoring its essential role in lens biology.
• The three-dimensional structure of beta-crystallin B1 has been determined, providing a framework for understanding its resilience against aggregation.
• An eye lens-specific protein, CRYBB1 expression is largely restricted to lens fiber cells, with no significant extra-lenticular expression.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the full-length human CRYBB1 protein (residues 1-252), suggesting that the antibody may recognize the native conformation in addition to denatured forms; conditions for reducing and non-reducing SDS-PAGE should be optimized.
• For Western blotting, a band of approximately 28 kDa is expected in human and mouse lens lysates; load appropriate positive and negative controls.
• In ELISA applications, consider using recombinant CRYBB1 as a standard and titrate the antibody to determine optimal working concentrations.
• As a polyclonal antibody, lot-to-lot variability is anticipated; validate each new lot in the intended assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering a broad portfolio of validated and customizable antibodies, including polyclonal, monoclonal, and antibody pairs, as well as ancillary reagents essential for diagnostic development. For researchers and manufacturers focusing on eye lens proteins or cataract biomarker discovery, CamelBio can source or produce CRYBB1-specific antibodies in bulk, along with technical consultation from concept to clinic.