Anti-CRYAB Rabbit Monoclonal Antibody for IHC-P, IF/ICC, ELISA - P02511

Core Product Specifications and Parameters

Biological Background: Alpha-crystallin B chain Function and Localization

• Alpha-crystallin B chain (CRYAB), also known as HSPB5, is a member of the small heat shock protein (sHSP) family and acts as a molecular chaperone, preventing aggregation of partially denatured proteins under stress conditions.
• It is a major structural protein in the eye lens, contributing to lens transparency and refractive index, and also stabilizes the vacuolar-type H(+)-ATPase protein ATP6V1A in lens epithelial cells.
• CRYAB is expressed in various tissues, notably in the lens and myocardial tissue, and its dysregulation is associated with diseases such as cataract and cardiomyopathy.
• The protein exists in multiple isoforms due to post-translational modifications including phosphorylation, acetylation, and oxidation, which may regulate its chaperone activity and subcellular localization.
• Subcellularly, CRYAB is found in the cytoplasm and nucleus, and can be secreted or localized to lysosomes under specific conditions.
• It functions as a metal-binding protein, with zinc ions potentially influencing its structure and function.
• Alpha-crystallin B chain is involved in the pathology of myofibrillar myopathy and other protein aggregation disorders, highlighting its role in protein quality control.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide covering amino acids 76–175 of human CRYAB, which is a central region conserved across species, explaining the reported cross-reactivity with rat.
• For immunohistochemistry (IHC-P) on paraffin-embedded tissues, consider testing on lens or myocardial sections and optimize antigen retrieval protocols, as CRYAB can be in cytosolic or nuclear compartments.
• In immunofluorescence/immunocytochemistry (IF/ICC), validate the antibody in relevant cell lines such as human lens epithelial cells or cardiomyocytes, and use appropriate fixation and permeabilization steps.
• For ELISA applications, pair this monoclonal antibody with another clone or a polyclonal detection antibody to build a sandwich assay, using recombinant CRYAB protein or cell lysates as standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, like this CRYAB rabbit monoclonal antibody, ideal for studying chaperone biology and lens-associated diseases. Our portfolio includes validated antibody pairs, bulk reagents, and customized sourcing for rare or niche targets in the heat shock protein family. From early-stage research to clinical assay development, CamelBio supports your project with reliable, high-quality raw materials and expert technical consultation.