Anti-CRYAB Polyclonal Antibody for WB and ELISA - P02511

Core Product Specifications and Parameters

Biological Background: Alpha-crystallin B chain Function and Localization

• Alpha-crystallin B chain (CRYAB), also known as HspB5, is a member of the small heat shock protein family. It is also referred to as renal carcinoma antigen NY-REN-27 and Rosenthal fiber component.
• Functions as a molecular chaperone, preventing the aggregation of various proteins under stress conditions. It may contribute to the transparency and refractive index of the lens.
• Belongs to the heat shock protein family B (small HSP) and is specifically classified as HSPB5.
• Subcellularly localizes to the cytoplasm, nucleus, secreted, and lysosome compartments, indicating diverse functional roles.
• Expressed in the lens as well as other tissues (PubMed:2387586, PubMed:838078). Also expressed in myocardial tissue (PubMed:28493373). Related references: PMID:2387586 PMID:838078 PMID:28493373
• Undergoes various post-translational modifications including phosphorylation, acetylation, and oxidation. It is associated with several human diseases such as cardiomyopathy, cataract, and myofibrillar myopathy.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a dilution range of 1:500-1:2000, with further optimization based on target abundance and sample type.
• Validate cross-reactivity in your specific mouse or rat tissue samples, as reactivity may vary with tissue preparation and expression levels.
• For ELISA, titrate the antibody to determine the optimal coating concentration suitable for your assay format.
• As a polyclonal antibody, verify lot-to-lot consistency for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This CRYAB antibody supports research in crystallin biology and stress response pathways. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development.