Anti-CRYAA Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P02489

Core Product Specifications and Parameters

Biological Background: Alpha-crystallin A chain Function and Localization

• Alpha-crystallin A chain, also known as heat shock protein beta-4 (HspB4), is a major structural protein of the vertebrate eye lens, contributing to its transparency and refractive index.
  Related references: PMID:18302245
• In its oxidized form, it acts as a molecular chaperone, preventing aggregation of denatured proteins under various stress conditions, a function critical for maintaining lens clarity.
  Related references: PMID:18199971, PMID:19595763, PMID:22120592
• CRYAA is required for the proper formation of lens intermediate filaments as part of a complex with BFSP1 and BFSP2.
  Related references: PMID:28935373
• It is a member of the small heat shock protein (sHSP) family and is predominantly expressed in the eye lens at the protein level.
• Subcellular localization includes both cytoplasm and nucleus.
• Post-translational modifications include acetylation, phosphorylation, oxidation, and disulfide bond formation, which may regulate its chaperone activity.
• Mutations in CRYAA are associated with autosomal dominant cataract, highlighting its importance in lens physiology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CRYAA protein (amino acids 1-173), which may allow broad recognition of both native and denatured forms. For Western blot, a specific band around 20 kDa should be detected in positive samples; consider using a recombinant protein control for verification.
• Cross-reactivity is confirmed with human, mouse, rat, and zebrafish; therefore, the antibody is suitable for cross-species studies. However, validation in your specific model system is recommended.
• For immunofluorescence (IF-P), permeabilization of cells or tissue sections is likely required to detect cytoplasmic and nuclear CRYAA. Optimization of fixation and permeabilization conditions is advised.
• In ELISA applications, the antibody may be used for antigen capture or detection. Pairing with a matched secondary antibody or detection system should be empirically optimized.
• Because CRYAA may exist in both reduced and oxidized forms under different conditions, consider sample preparation conditions (e.g., reducing vs. non-reducing) to probe specific redox states.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supplier, CamelBio offers reliable access to this CRYAA polyclonal antibody and a wide range of ancillary reagents for lens biology and chaperone research. In addition to bulk antibody orders, we can assist in sourcing validated antibody pairs, optimized monoclonal antibodies, and critical raw materials to accelerate your diagnostic or research workflow. Partner with us to navigate the full development cycle, from concept to clinic.