Anti-CRYAA Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P02489

Core Product Specifications and Parameters

Biological Background: CRYAA Function and Localization

• Alpha-crystallin A chain (CRYAA), also known as HspB4, is a member of the small heat shock protein family and a major structural protein of the vertebrate eye lens.
• Contributes to lens transparency and refractive index, essential for proper vision. Related references: PMID:18302245
• Functions as a molecular chaperone, preventing aggregation of various proteins under stress conditions when in its oxidized (non-disulfide-bonded) form. Related references: PMID:18199971 PMID:19595763 PMID:22120592
• Required for the correct formation of lens intermediate filaments, forming a complex with BFSP1 and BFSP2. Related references: PMID:28935373
• Localizes to both the cytoplasm and nucleus, consistent with its roles in protein homeostasis and lens fiber cell differentiation.
• Tissue-specific expression at the protein level in the eye lens; mutations in CRYAA are associated with congenital cataracts.
• Post-translational modifications include acetylation, phosphorylation, oxidation, and disulfide bond formation, which modulate its chaperone activity and structural stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 6–95, which covers a conserved N-terminal region; confirm reactivity with your target species (human, mouse, rat) using positive control lysates.
• For Western blot, CRYAA migrates at approximately 20 kDa under reducing conditions; consider non-reducing conditions if disulfide-mediated complexes are of interest.
• IF-P staining is likely to show cytoplasmic and nuclear distribution; validate with a lens-derived cell line or tissue sections.
• The antibody is also suitable for ELISA-based quantification; optimize coating and detection conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined, one-stop supply of IVD raw materials. For projects targeting lens proteins or chaperone-related biomarkers, we can source validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents. Whether you need this anti-CRYAA clone for a vision-research kit or custom-sourced rare-target antibodies, CamelBio bridges the gap from concept to clinic.