Anti-CRY2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q49AN0

Core Product Specifications and Parameters

Biological Background: CRY2 Function and Localization

• Cryptochrome-2 (CRY2) is a core component of the circadian clock, acting as a transcriptional repressor in the negative limb of the transcription/translation feedback loop (TTFL). It interacts with the CLOCK/BMAL1 heterodimer to inhibit its transcriptional activity.
• Together with CRY1, CRY2 regulates the circadian period; while both have redundant functions, CRY2 has a less potent repressor activity in cerebellum and liver and plays a critical role in tuning the SCN circadian period by opposing CRY1 actions.
• CRY2 is involved in glucose and lipid metabolism modulation, repressing PPARD target genes in skeletal muscle, and regulating genes like LEP and ACSL4.
• It represses glucocorticoid receptor (NR3C1/GR)-induced transcriptional activity by binding to glucocorticoid response elements, and also represses CLOCK-BMAL1 induced transcription of BHLHE40/DEC1 and NAMPT.
• Subcellularly, CRY2 localizes to both the cytoplasm and nucleus, allowing it to shuttle between compartments as part of clock regulation.
• Post-translational modifications, including phosphorylation and ubiquitination, are critical for determining the circadian period (tau) and stability of CRY2.
• CRY2 is widely expressed in human tissues, with highest levels in heart and skeletal muscle, and also present in brain, liver, kidney, and many others.
• The protein binds FAD and may have photoreceptive properties, though in mammals its primary role is transcriptional regulation within the clock mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 455-614), which may be well-exposed under denaturing conditions; consider using reducing SDS-PAGE for Western blot to detect the 67kDa band.
• For immunofluorescence/ICC, optimize fixation and permeabilization protocols, as CRY2 localizes to both cytoplasm and nucleus; methanol or paraformaldehyde with Triton X-100 may yield different results.
• ELISA can be used for quantification; consider a sandwich ELISA approach with a matched antibody pair, and validate linearity and sensitivity using recombinant CRY2 protein.
• Cross-reactivity is confirmed for human and rat samples; when using other species, run a sequence alignment and validate in the relevant sample system.
• Include appropriate positive controls (e.g., HEK293, HeLa cell lysates) and negative controls (siRNA knockdown or knockout samples) to confirm antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. For circadian clock and transcription research, this anti-CRY2 antibody complements our portfolio of core clock component antibodies and assay development tools. As a trusted sourcing partner, CamelBio streamlines procurement from concept to clinic, offering bulk supply, custom formulation, and technical support to accelerate your diagnostic or research projects.