Anti-CRY2 Rabbit Polyclonal Antibody for WB, ELISA - Q49AN0

Core Product Specifications and Parameters

Biological Background: CRY2 Function and Localization

• CRY2 (Cryptochrome-2) is a transcriptional repressor and core component of the circadian clock, mediating ~24-hour rhythms in gene expression to regulate metabolism, sleep, and other physiological processes.
• It operates in a transcription/translation feedback loop (TTFL) with CLOCK/BMAL1 heterodimers: CRY2 represses CLOCK-BMAL1 activity, thereby inhibiting its own expression and that of PER genes.
• Along with CRY1, it has redundant but distinct roles; CRY2 tunes the circadian period in the suprachiasmatic nucleus (SCN) by opposing CRY1 action, and is essential for intercellular rhythm synchrony.
• Beyond the core clock, CRY2 modulates glucose and lipid metabolism by regulating genes like LEP and ACSL4, represses glucocorticoid receptor signaling, and influences cAMP pathways.
• Subcellular localization: CRY2 shuttles between the cytoplasm and nucleus, consistent with its role in transcriptional regulation.
• Tissue specificity: ubiquitously expressed, with highest levels detected in heart and skeletal muscle.
• Keywords: Photoreceptor protein, FAD-binding flavoprotein, chromophore, phosphoprotein, Ubl conjugation, alternative splicing, and biological rhythms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (aa 200-300) of human CRY2, which may explain the reported cross-reactivity with mouse and rat. Validate reactivity in your specific model system.
• For Western blot, expect a band at approximately 67 kDa; lysates from heart or skeletal muscle may yield higher endogenous expression.
• For ELISA, consider using the recombinant immunogen as a positive control, or optimize coating with tissue/cell lysates to detect native CRY2.
• Given the nuclear and cytoplasmic localization, thorough lysis is recommended for total protein extraction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined one-stop channel for validated IVD raw materials, including optimized antibodies, ancillary reagents, and custom sourcing. This anti-CRY2 polyclonal antibody supports circadian rhythm and metabolic research applications. Contact us to explore bulk options or complementary reagents for your assay development needs.