Anti-CRY1 Polyclonal Antibody for WB, IHC-P, ELISA - Q16526

Core Product Specifications and Parameters

Biological Background: CRY1 Function and Localization

• CRY1 (Cryptochrome-1) is a transcriptional repressor and a core component of the circadian clock, forming the negative limb of the transcription/translation feedback loop (TTFL). It heterodimerizes with PER proteins to interact with and inhibit the CLOCK/BMAL1 complex.
• The circadian clock governs ~24-hour rhythms in gene expression, regulating metabolism, sleep, body temperature, and many physiological processes. CRY1, along with CRY2, PER1-3, and CLOCK/BMAL1, drives this molecular oscillator.
• CRY1 and CRY2 have redundant yet distinct functions: CRY1 is a more potent transcriptional repressor in cerebellum and liver, while CRY2 tunes the period of the SCN oscillator. CRY1 is essential for intercellular network development and rhythm synchrony.
• Subcellular localization: CRY1 is found in both the cytoplasm and nucleus. It can translocate clock proteins (e.g., PER) to the nucleus and act as a gatekeeper for CLOCK-BMAL1 at DNA-binding sites.
• Post-translational modifications: CRY1 undergoes phosphorylation and is a chromophore-binding flavoprotein (FAD). It is also a photoreceptor protein involved in light entrainment of the circadian clock.
• Beyond the clock, CRY1 represses glucocorticoid receptor NR3C1/GR transcriptional activity and plays a role in glucose and lipid metabolism. In liver, it mediates circadian regulation of cAMP signaling and gluconeogenesis, inhibiting FOXO1 and CREB1 pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (aa 496-586) of human CRY1; this region may be less conserved between CRY1 and CRY2, potentially offering isoform specificity. Validate in relevant sample systems (e.g., SCN-containing brain extracts, liver lysates) with positive and negative controls.
• This antibody is suitable for Western blotting (WB), immunohistochemistry-paraffin (IHC-P), and ELISA. For WB, a band near 66 kDa is expected; consider loading controls such as GAPDH or β-actin. For IHC-P, appropriate antigen retrieval and permeabilization steps may be required due to nuclear localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, encompassing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our product portfolio includes this high-quality anti-CRY1 antibody for circadian rhythm research and related diagnostic applications. Additionally, we can support the sourcing of rare targets and customized raw materials to accelerate your assay development from concept to clinic.