Anti-CRTC1 Rabbit Polyclonal Antibody for WB and ELISA - Q6UUV9

Core Product Specifications and Parameters

Biological Background: CRTC1 Function and Localization

• CRTC1 (CREB-regulated transcription coactivator 1), also known as MECT1 or TORC1, is a transcriptional coactivator that enhances CREB1-mediated transcription at cAMP response element (CRE) sites, functioning in the SIK/TORC signaling pathway.
• It acts independently of CREB1 Ser-133 phosphorylation, enhancing CREB1 interaction with TAF4, and regulates specific genes like StAR and PGC1alpha, thereby inducing mitochondrial biogenesis.
• In the hippocampus, CRTC1 is required for late-phase long-term potentiation (L-LTP) maintenance at Schaffer collateral-CA1 synapses and may support dendritic growth in developing cortical neurons (By similarity).
• Together with SIK1, it regulates light-induced entrainment of the circadian clock by coactivating CREB-mediated transcription of PER1.
• CRTC1 serves as a coactivator for the HTLV-1 Tax protein, enhancing viral LTR transcription during microbial infection.
• The protein localizes to both cytoplasm and nucleus and is subject to alternative splicing and phosphorylation; it is highly expressed in adult and fetal brain, especially prefrontal cortex and cerebellum, with very low expression elsewhere.
• Chromosomal rearrangements involving CRTC1 are associated with mucoepidermoid carcinoma.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–200 of human CRTC1 (NP_056136.2), covering the N-terminal region. This design may be suitable for detecting full-length CRTC1 in WB; consider using brain or neuronal cell lysates due to high expression.
• For ELISA, the antibody may be used for direct or indirect detection; optimal working concentrations should be determined empirically.
• Since this is a polyclonal antibody, lot-to-lot variability can occur; validate in your specific sample system, especially when comparing expression across species (cross-reactivity with mouse and rat is indicated, but confirm in your assay).
• CRTC1 undergoes phosphorylation, which may affect antibody recognition; consider dephosphorylation assays or phosphatase inhibitors if targeting specific phospho-states.

CamelBio: Your One-Stop Sourcing Bridge

As a specialist in IVD raw materials, CamelBio offers a comprehensive portfolio including validated antibody pairs, recombinant proteins, and ancillary reagents. For your CRTC1-related research in transcription regulation and neurological disorders, we provide bulk quantities and support for custom sourcing needs. Partner with CamelBio to streamline your transition from concept to clinical application.