Anti-CROT Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9UKG9

Core Product Specifications and Parameters

Biological Background: CROT Function and Localization

• CROT, also known as COT, is a peroxisomal carnitine O-octanoyltransferase that catalyzes the transfer of acyl groups from coenzyme A to carnitine, facilitating the transport of fatty acids across peroxisomal membranes.
• It plays a key role in the beta-oxidation of fatty acids, with the highest enzymatic activity toward medium-chain substrates (C6 to C10).
• CROT converts 4,8-dimethylnonanoyl-CoA, the end product of pristanic acid beta-oxidation, into its carnitine ester, enabling further metabolism or export.
• The enzyme is localized exclusively to peroxisomes, the organelles responsible for fatty acid breakdown and hydrogen peroxide detoxification.
• CROT belongs to the carnitine acyltransferase family, which includes enzymes like CPT1 and CPT2, and is essential for lipid homeostasis.
• Post-translational modifications such as acetylation have been identified, though their functional impact remains to be fully characterized.
• It participates in intracellular transport processes by shuttling peroxisomal metabolites, linking lipid metabolism with overall cellular energy regulation.
• Alternative splicing generates multiple isoforms, which may have tissue-specific roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-87) of human CROT, suggesting the antibody may recognize an epitope in this domain. Consider verifying specificity with recombinant protein or knockout controls.
• For Western blot (WB), a band around 70 kDa is expected based on the calculated molecular weight; however, post-translational modifications or isoform variation may cause slight shifts. Use appropriate reducing/denaturing conditions.
• The antibody cross-reacts with mouse and rat CROT, but sequence alignment is recommended if working with other species.
• For immunoprecipitation (IP), optimize antibody concentration and incubation conditions to avoid non-specific binding.
• ELISA performance has been noted; consider using standard sandwich ELISA protocols with appropriate blocking to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CROT polyclonal antibody supports research in fatty acid metabolism and peroxisomal disorders, aligning with our extensive portfolio of lipid metabolism-related targets. From validated antibody pairs to bulk ancillary reagents, CamelBio streamlines sourcing to accelerate your assay development.