Anti-CRMP2/DPYSL2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q16555

Core Product Specifications and Parameters

Biological Background: CRMP2/DPYSL2 Function and Localization

• Dihydropyrimidinase-related protein 2 (DRP-2), also known as collapsin response mediator protein 2 (CRMP-2) or ULP-2, is a member of the CRMP family of cytosolic phosphoproteins involved in neuronal development and polarity.
• CRMP2 plays a critical role in axon growth and guidance, neuronal growth cone collapse, and cell migration, functioning downstream of class 3 semaphorin signaling to remodel the cytoskeleton.
• The protein is implicated in endocytosis, suggesting a broader role in membrane trafficking events beyond cytoskeletal regulation.
• Subcellular localization includes the cytoplasm, cytosol, cytoskeleton, and membrane, consistent with its dynamic roles in neuronal cell architecture.
• Expression is ubiquitous across tissues, with prominent roles in the nervous system during development and in mature neuronal functions.
• Post-translational modifications, including phosphorylation, methylation, and S-nitrosylation, modulate CRMP2 activity and interactions, contributing to its regulatory versatility.
• Alternative splicing produces multiple isoforms, potentially diversifying its functional repertoire in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 400–500 of human CRMP2/DPYSL2; consider this region when predicting epitope specificity or designing confirmatory assays.
• For western blotting, an expected band at ~62 kDa may be detected in whole-cell lysates; validate band size using appropriate controls and consider phosphatase inhibitors if phosphorylation states are relevant.
• In immunohistochemistry (paraffin-embedded tissues), optimize antigen retrieval and antibody dilution for the specific tissue and fixation conditions; endogenous peroxidase blocking is recommended for HRP-based detection.
• For ELISA applications, the immunizing peptide may serve as a positive control; test direct and sandwich formats with appropriate blocking agents to minimize background.
• Cross-reactivity with mouse and rat samples is supplier-specified; independently verify reactivity and specificity in your target species using lysates or tissue sections.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-CRMP2/DPYSL2 rabbit polyclonal antibody, targeting a key neuronal development protein, exemplifies our commitment to supplying high-quality research reagents. Through our integrated platform, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your diagnostic and research workflows.