Anti-CRMP2/DPYSL2 Monoclonal Antibody for WB, IF-P, ELISA - Q16555

Core Product Specifications and Parameters

Biological Background: CRMP2/DPYSL2 Function and Localization

• Dihydropyrimidinase-related protein 2 (CRMP2/DPYSL2), also known as collapsin response mediator protein 2, N2A3, unc-33-like phosphoprotein 2 (ULIP-2), is encoded by the DPYSL2 gene in humans.
• Plays a central role in neuronal development and polarity, axon growth and guidance, growth cone collapse, and cell migration. It is necessary for signaling by class 3 semaphorins and subsequent cytoskeletal remodeling.
• May also participate in endocytosis.
• Subcellular localization includes cytosol, cytoskeleton, and membrane.
• Ubiquitously expressed across tissues.
• Subject to multiple post-translational modifications; annotated as a phosphoprotein, methylated, and S-nitrosylated. Involved in neurogenesis and differentiation, with alternative splicing generating isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 473–572 of human CRMP2/DPYSL2; consider using full-length protein or the relevant peptide for competitive assays if needed.
• For WB, a band at ~62 kDa is expected; validate in neuronal cell lines or tissues from human, mouse, or rat.
• For IF-P, permeabilization is required for intracellular detection; cytoskeletal staining may be observed.
• Optimize antibody dilution for each application and sample type through preliminary titration experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated antibodies like anti-CRMP2/DPYSL2. For neuronal development and axon guidance research, we offer customized monoclonal/polyclonal antibody development, bulk reagent supply, and sourcing of rare targets. Accelerate your assay development with our integrated technical and supply chain support.