Anti-CRMP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14194

Core Product Specifications and Parameters

Biological Background: CRMP1 Function and Localization

• Dihydropyrimidinase-related protein 1 (CRMP1), also known as Collapsin response mediator protein 1 (CRMP-1) or Unc-33-like phosphoprotein 3 (ULIP-3), belongs to the CRMP family of cytosolic phosphoproteins.
• Plays a necessary role in signaling by class 3 semaphorins, particularly SEMA3A, leading to cytoskeleton remodeling and axon guidance; acts downstream of SEMA3A to promote FLNA dissociation from F-actin, resulting in growth cone collapse. Related references: PMID:25358863
• Involved in invasive growth and cell migration, suggesting a broader role in cellular motility beyond the nervous system. Related references: PMID:11562390
• May participate in cytokinesis, the final stage of cell division. Related references: PMID:19799413
• Localizes to multiple cytoplasmic compartments, including the cytoskeleton, centrosome, spindle, growth cone, and perikaryon, reflecting its dynamic roles in cell structure.
• Predominantly expressed in the brain, consistent with its function in neuronal development and axon guidance.
• Subject to post-translational modifications such as phosphorylation and nitration, which may regulate its activity and interactions.
• Undergoes alternative splicing, producing protein variants with potentially distinct functions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 460–540 near the C-terminus; confirm that the antibody recognizes the isoforms relevant to your experimental system.
• For Western blot (WB), a band at approximately 62 kDa is expected; use appropriate positive controls (e.g., brain lysates) and consider blocking conditions optimised for polyclonal antibodies.
• In immunofluorescence/immunocytochemistry (IF/ICC), detection of cytoplasmic and cytoskeletal targets may require mild permeabilisation; validate fixation protocols for your cell type.
• Cross-reactivity is reported for human, mouse, and rat; however, we recommend verifying reactivity in your specific samples, especially if using less common strains or tissues.
• For ELISA, consider pairing with a suitable capture/detection antibody system, and perform checkerboard titrations to determine optimal concentrations.
• Since CRMP1 is a signalling regulator, ensure that treatments (e.g., SEMA3A stimulation) are carefully timed to capture dynamic localisation changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio proudly presents this CRMP1 polyclonal antibody as part of our comprehensive IVD raw-material portfolio designed for neuroscience and cell motility research. Beyond this product, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your diagnostic and research workflows. Trust CamelBio for reliable, one-stop access to the critical building blocks you need, from concept to clinic.