Anti-CRMP1 Rabbit Monoclonal Antibody for WB and ELISA - Q14194

Core Product Specifications and Parameters

Biological Background: CRMP1 Function and Localization

• CRMP1, also known as dihydropyrimidinase-related protein 1 (DRP1) or collapsin response mediator protein 1 (CRMP-1), is encoded by the CRMP1 gene (DPYSL1, ULIP3) and belongs to the dihydropyrimidinase-related protein family.
• It functions as a key mediator in class 3 semaphorin signaling, particularly SEMA3A, to regulate axon guidance and growth cone collapse by promoting FLNA dissociation from F-actin, leading to cytoskeletal rearrangement (PubMed:25358863). Related references: PMID:25358863
• CRMP1 is involved in invasive growth and cell migration, contributing to cellular processes beyond the nervous system (PubMed:11562390). Related references: PMID:11562390
• The protein may also play a role in cytokinesis, suggesting functions in cell division (PubMed:19799413). Related references: PMID:19799413
• CRMP1 is predominantly expressed in the brain, aligning with its established role in neuronal development.
• Subcellularly, CRMP1 localizes to the cytoplasm, centrosome, mitotic spindle, growth cones, and perikaryon, consistent with its cytoskeletal regulatory roles.
• Post-translational modifications include phosphorylation and nitration, and the gene undergoes alternative splicing.
• The predicted molecular weight is approximately 62 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 450–550 of human CRMP1. Verify sequence conservation in rat to ensure expected cross-reactivity.
• For Western blot, a band migrating around 62 kDa is anticipated. Include appropriate brain‑tissue lysates as positive controls.
• In ELISA, titrate both the primary antibody and detection reagents, and consider using synthetic peptide standards to assess linear range and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. This CRMP1 rabbit monoclonal antibody is a valuable tool for studying axon guidance, cell migration, and cytoskeletal dynamics. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and flexible sourcing for rare-target raw materials, enabling seamless procurement from discovery through clinical development.