Anti-CRM1/XPO1 Rabbit Polyclonal Antibody for WB and ELISA - O14980

Core Product Specifications and Parameters

Biological Background: Exportin-1 Function and Localization

• Exportin-1 (CRM1/XPO1) is a nuclear export receptor that mediates the nucleocytoplasmic transport of proteins and RNAs bearing a leucine-rich nuclear export signal (NES).
• In the nucleus, it cooperatively binds NES-containing cargo and Ran-GTP, facilitated by RANBP3, forming a trimeric export complex.
• The export complex docks at the nuclear pore complex via nucleoporins, translocates to the cytoplasm, and disassembles upon Ran-GTP hydrolysis, releasing the cargo.
• CRM1 is responsible for the nuclear export of diverse cellular proteins and also mediates U3 snoRNA transport from Cajal bodies to nucleoli.
• It plays a critical role in viral replication by exporting unspliced or incompletely spliced viral RNAs from HIV-1, HTLV-1, and influenza A, interacting with viral proteins like HIV-1 Rev and HTLV-1 Rex.
• Subcellular localization includes the cytoplasm, nucleoplasm, Cajal bodies, and nucleolus, reflecting its function in multiple nuclear export pathways.
• CRM1 is broadly expressed across human tissues (heart, brain, lung, liver, etc.) but is notably absent in the kidney.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity, and it contains repeat domains and RNA-binding capability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved C‑terminal region (aa 964–1064) of human CRM1, supporting cross‑reactivity with mouse; confirm specificity by peptide competition assay.
• For Western blot, expect a band at ~123 kDa; optimize blocking conditions and antibody dilution for large‑molecular‑weight proteins.
• ELISA applications may require peptide coating or a capture‑antibody setup; validate in the relevant sample matrix.
• Consider nuclear/cytoplasmic fractionation to enrich CRM1 signals when studying subcellular distribution.
• As a nuclear export receptor, CRM1 function can be assessed using appropriate transport inhibitors as controls in functional assays.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and bulk ancillary reagents. Our CRM1/XPO1 antibody supports nuclear transport and virology research, with options for custom scale‑up and technical consultation. From concept to clinic, CamelBio bridges your diagnostic development needs with reliable one‑stop sourcing.