Anti-CRLS1 Polyclonal Antibody for WB, ELISA - Q9UJA2

Core Product Specifications and Parameters

Biological Background: CRLS1 Function and Localization

• CRLS1 encodes cardiolipin synthase (CMP-forming), also known as Protein GCD10 homolog, a mitochondrial enzyme responsible for the final step of cardiolipin biosynthesis.
• The enzyme catalyzes the transfer of a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol to form cardiolipin (diphosphatidylglycerol). Related references: PMID:16547353, PMID:16678169, PMID:16716149
• Cardiolipin is a key component of mitochondrial membranes, essential for maintaining functional integrity and dynamics, especially under stress. Related references: PMID:35147173
• CRLS1 localizes to the mitochondrial inner membrane, where cardiolipin is synthesized.
• The gene is highly expressed in heart, skeletal muscle, and liver tissues with high mitochondrial content.
• Alternative splicing produces multiple transcript variants, indicating potential isoform diversity.
• UniProt classifies CRLS1 under disease variant and primary mitochondrial disease keywords, linking it to mitochondrial disorders.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 110 amino acids of human CRLS1, which may allow the antibody to recognize full-length CRLS1 and potentially N-terminal isoforms in human samples.
• For Western blot, a band around 33 kDa is expected based on the predicted protein weight; however, actual migration may vary due to post-translational modifications or isoform expression.
• ELISA can be used for quantitative detection of CRLS1, but appropriate blocking and antigen coating conditions should be optimized.
• Cross-reactivity is reported only for human; for other species, sequence homology should be checked and performance validated experimentally.
• As a polyclonal antibody, it may exhibit batch-to-batch variation; consider validation with relevant positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-CRLS1 pAb, supporting mitochondrial research and lipid metabolism studies. We provide bulk ancillary reagents and rare target sourcing to streamline your diagnostic development from concept to clinic.