Anti-CRLF2 Rabbit Polyclonal Antibody for WB, ELISA - Q9HC73

Core Product Specifications and Parameters

Biological Background: CRLF2 Function and Localization

• CRLF2, also known as cytokine receptor-like factor 2 or thymic stromal lymphopoietin receptor (TSLPR), is a receptor for thymic stromal lymphopoietin (TSLP).
• It forms a functional heterodimeric complex with TSLP and IL7R (interleukin-7 receptor alpha chain), initiating downstream signaling.
• The receptor complex signals through JAK2 and activates STAT3 and STAT5 transcription factors, promoting cell proliferation.
• CRLF2 plays a critical role in the development of the hematopoietic system.
• The protein is primarily localized to the cell membrane; an alternatively spliced secreted isoform has also been identified.
• Tissue expression is broad, found in heart, skeletal muscle, kidney, and liver, with primary expression in dendritic cells and monocytes.
• Post-translational modifications include disulfide bonds and glycosylation, contributing to its structural stability and function.
• The canonical protein has a calculated molecular weight of approximately 42 kDa (42013 Da).

Experimental Guidance and Technical Tips

• The immunogen (aa 23-231) targets the N-terminal extracellular domain; consider its suitability for detecting both full-length and secreted isoforms.
• For Western blot, expect a band around 42 kDa under reducing conditions; validation with positive controls (e.g., dendritic cell lysates) is recommended.
• In ELISA applications, optimize coating antigen and antibody dilution, and account for cross-reactivity with mouse and rat CRLF2 when selecting detection reagents.
• If employing this antibody in untested applications (e.g., IHC, FC), perform thorough specificity validation due to potential polyclonal batch variation.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated supplier of IVD raw materials, CamelBio streamlines your procurement from discovery to commercialization. Complementing this CRLF2 antibody, we offer validated antibody pairs, optimized recombinant proteins, and ancillary reagents for cytokine receptor research. Our team assists with custom sourcing for rare targets and bulk orders, ensuring consistent quality for high-throughput diagnostic development.