Anti-CRKL Rabbit Polyclonal Antibody for WB, ELISA - P46109

Core Product Specifications and Parameters

Biological Background: Crk-like protein Function and Localization

• CRKL (Crk-like protein) is an adaptor protein that mediates the transduction of intracellular signals.
• The protein contains SH2 and SH3 domains, which facilitate protein-protein interactions in signaling pathways.
• It is classified as a phosphoprotein and is subject to post-translational phosphorylation, regulating its activity and binding partners.
• UniProt keywords include "3D-structure" and "Reference proteome," indicating extensive structural and proteomic characterization.
• The official gene name is CRKL, and its molecular weight is approximately 34 kDa (calculated from sequence: ~33.8 kDa).

Experimental Guidance and Technical Tips

• The immunogen (full-length human CRKL, aa 1-303) suggests the antibody recognizes native and denatured forms, supporting WB and ELISA applications.
• For WB, consider testing a range of antibody dilutions (e.g., 1:500–1:2000) and validating with positive and negative cell lysates.
• Cross-reactivity with mouse and rat is confirmed; due to high sequence homology, reactivity with other species may be possible but should be verified experimentally.
• ELISA optimization may require checkerboard titrations of coating antigen and antibody concentrations.
• Always include appropriate controls (e.g., recombinant CRKL protein, knockdown samples) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic manufacturers and research laboratories. For CRKL-related signal transduction studies, CamelBio can supply this polyclonal antibody alongside complementary reagents to accelerate assay development. Partner with us to streamline your supply chain from concept to clinic.