Anti-CRKL Polyclonal Antibody for WB, ELISA - P46109

Core Product Specifications and Parameters

Biological Background: Crk-like protein Function and Localization

• CRKL (Crk-like protein) is an adapter protein containing SH2 and SH3 domains, facilitating protein-protein interactions in intracellular signaling cascades.
• It may mediate transduction of intracellular signals, likely functioning downstream of receptor tyrosine kinases, though its full biological roles remain under investigation.
• The protein features one SH2 domain that binds phosphotyrosine-containing motifs and two SH3 domains (N-terminal and C-terminal) that recognize proline-rich sequences, enabling assembly of multi-protein complexes.
• Annotated as a phosphoprotein in UniProt, CRKL is subject to phosphorylation events that can regulate its activity and interactions.
• The human CRKL gene (Gene ID: 1399) encodes a protein with a calculated molecular weight of approximately 33.8 kDa, consistent with the observed 34 kDa band in Western blot.
• CRKL is part of the Crk adaptor protein family, which plays roles in cell proliferation, migration, and differentiation.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a specific band at ~34 kDa in lysates from human and mouse cells/tissues. Optimize antibody dilution empirically; typical starting dilutions range from 1:500 to 1:2000 for polyclonal antibodies.
• For ELISA, the immunogen peptide (amino acids 201-300) can be used as a coating antigen for indirect ELISA to determine antibody titer. For sandwich ELISA, pair with a validated capture antibody if available.
• Since this is a polyclonal antibody, it may exhibit lot-to-lot variability; we recommend validating each new lot in the intended application.
• The antibody cross-reacts with human and mouse CRKL, but reactivity with other species is not guaranteed; verify in the species of interest.
• For immunohistochemistry or other applications not listed, performance must be tested by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-CRKL antibody supports signal transduction studies and assay development, and is part of our broader portfolio covering a wide range of targets. We assist with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your diagnostic projects.