Anti-CRKL (Phospho-Tyr207) Rabbit Polyclonal Antibody for WB, ELISA - P46109

Core Product Specifications and Parameters

Biological Background: CRKL Function and Localization

• CRKL (Crk-like protein) is a 34 kDa adaptor protein containing SH2 and SH3 domains, which facilitate protein-protein interactions in signal transduction.
• It is classified as a phosphoprotein, suggesting its activity and interactions may be regulated by phosphorylation events.
• CRKL likely mediates intracellular signal transduction, though specific pathways are not fully delineated in the current UniProt record.
• The protein includes repeating structural motifs, as indicated by the keyword 'Repeat' in its UniProt entry.
• It is part of the reference proteome, marking its inclusion in the human proteome project.
• The SH2 domain binds phosphotyrosine-containing motifs, while SH3 domains recognize proline-rich sequences, enabling assembly of signaling complexes.
• Information on subcellular localization and tissue-specific expression is not provided in the reviewed UniProt record.
• Additional functional details may emerge from further research.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide around Tyr207; validate phospho-specificity using phosphatase-treated samples or non-phospho peptide competition.
• For western blotting, expect a band around 34 kDa; optimize gel percentage and transfer conditions for proteins in this range.
• The antibody may be used for ELISA to detect phospho-CRKL (Tyr207) in cell or tissue lysates; optimize coating and detection conditions.
• With confirmed mouse cross-reactivity, the antibody can be applied to mouse samples, but validation in the relevant sample system is recommended.
• As a polyclonal antibody, lot-to-lot variability may occur; consider validating performance for each new lot.

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CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including phosphorylated antibodies like this anti-CRKL pAb. Our comprehensive portfolio covers validated antibody pairs, bulk ancillary reagents, and specialized raw-material sourcing for signal transduction research. We support every stage from concept to clinic, ensuring reliable supply for your diagnostic and translational needs.