Anti-CRK/CRKII Rabbit Monoclonal Antibody for WB, ELISA - P46108

Core Product Specifications and Parameters

Biological Background: CRK/CRKII Function and Localization

• Adapter molecule crk (also known as proto-oncogene c-Crk and p38) is a signaling adaptor that contains one SH2 and two SH3 domains, mediating protein-protein interactions.
• Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.
• Regulates cell adhesion, spreading and migration. Related references: PMID:31311869
• Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner.
• Functions in phagocytosis of apoptotic cells and cell motility through interactions with DOCK1 and DOCK4. Related references: PMID:19004829
• May regulate EFNA5-EPHA3 signaling (By similarity).
• Subcellular localization includes cytoplasm and cell membrane, consistent with its role in signal transduction from membrane receptors.
• Post-translational modifications include phosphorylation and acetylation; alternative splicing produces isoforms like CRKII.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human CRK/CRKII, common to both isoforms; thus, the antibody may detect CRK and CRKII.
• Recommended applications based on provider specifications: Western blot (WB) and ELISA. Consider validating in your specific experimental setup.
• Reported cross-reactivity with human and mouse samples; for other species, verify sequence homology of the immunogen region.
• As a rabbit monoclonal antibody, it offers consistent specificity and low background; however, optimal working dilutions should be empirically determined.
• Use appropriate positive and negative controls, and consider testing in relevant cell lines or tissues where CRK/CRKII is expressed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material procurement, supporting diagnostic manufacturers and research laboratories in acquiring high-quality antibodies and auxiliary reagents. For targets like CRK/CRKII, which play key roles in cell adhesion, migration, and cancer-related signaling, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials. Contact us to explore how we can streamline your development pipeline from concept to clinic.