Anti-CRK/CRKII Polyclonal Antibody for WB, ELISA - P46108

Core Product Specifications and Parameters

Biological Background: CRK/CRKII Function and Localization

• CRK, also known as adapter molecule crk or proto-oncogene c-Crk, is encoded by the CRK gene and exists as two major isoforms, CRKI and CRKII, through alternative splicing.
• CRK/CRKII functions as an adaptor protein containing SH2 and SH3 domains, linking tyrosine kinase signals to downstream effectors involved in cell adhesion, spreading, and migration.
• It regulates cell adhesion, spreading, and migration through BCAR1-CRK-RAPGEF1 signaling and RAP1 activation. Related references: PMID:31311869
• CRK mediates attachment-induced MAPK8 activation, membrane ruffling, and cell motility in a Rac-dependent manner.
• Through interactions with DOCK1 and DOCK4, CRK plays a role in phagocytosis of apoptotic cells and cell motility. Related references: PMID:19004829
• CRK may regulate EFNA5-EPHA3 signaling.
• Localizes to the cytoplasm and cell membrane, consistent with its role in signal transduction at the plasma membrane.
• Post-translational modifications include phosphorylation and acetylation; as a proto-oncogene, its dysregulation may contribute to oncogenic processes.

Experimental Guidance and Technical Tips

• For Western blot detection, the predicted molecular weight of CRKII is 34 kDa; additional bands corresponding to CRKI (~23 kDa) or modified forms may be observed.
• Optimize antibody dilution and incubation conditions based on endogenous expression levels in your specific cell or tissue lysates.
• Validate cross-reactivity in mouse samples if required, and include appropriate positive and negative controls to confirm specificity.
• For ELISA, determine optimal coating and detection parameters empirically; the antibody may be used as capture or detection reagent in compatible assay formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This anti-CRK/CRKII polyclonal antibody supports research in cell adhesion, migration, and oncogenic signaling. Contact CamelBio for bulk orders and customized sourcing solutions.