Anti-CRISPLD2 Polyclonal Antibody for WB and ELISA - Q9H0B8

Core Product Specifications and Parameters

Biological Background: CRISPLD2 Function and Localization

• CRISPLD2 (Cysteine-rich secretory protein LCCL domain-containing 2) is also known as CRISP-11, LGL1, and CRISP11; it belongs to the CAP superfamily of cysteine-rich secretory proteins and contains an LCCL domain.
• The protein is encoded by the CRISPLD2 gene (GeneID: 83716) and undergoes alternative splicing, generating multiple isoforms.
• CRISPLD2 functions as a promoter of matrix assembly, likely contributing to extracellular matrix organization and tissue architecture.
• It is a secreted protein that localizes to the extracellular space, consistent with its role in matrix remodeling.
• CRISPLD2 contains multiple conserved cysteine residues forming intramolecular disulfide bonds, which are critical for its structural stability and function.
• Post-translational modifications include N-linked glycosylation, as indicated by the glycoprotein keyword, which may influence protein folding, stability, and secretion.
• CRISPLD2 is identified in proteomic analyses, supporting its presence in various biological contexts.
• The protein has a predicted molecular weight of approximately 56 kDa (55.9 kDa from UniProt), consistent with the immunogen design.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 140–430 of human CRISPLD2, covering a central region that includes the LCCL domain; this may allow recognition of multiple isoforms and processed forms.
• For Western blotting, a band around 56 kDa is expected; however, post-translational modifications (e.g., glycosylation) may shift the apparent molecular weight. Consider using a reducing SDS-PAGE protocol.
• Cross-reactivity with mouse and rat has been indicated; validate antibody performance in relevant species and tissue samples before routine use.
• For ELISA, this polyclonal antibody can be explored as both capture and detection reagent, but optimal pairing and concentrations should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to a wide range of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For matrix biology and extracellular matrix research, we supply high-quality CRISPLD2 antibodies and can source difficult-to-find targets to support your assay development from concept to clinic.