Anti-CRIP2 Rabbit Polyclonal Antibody for WB, ELISA - P52943

Core Product Specifications and Parameters

Biological Background: CRIP2 Function and Localization

• CRIP2 (Cysteine-rich protein 2), also designated ESP1, is a LIM domain-containing protein characterized by two tandem LIM zinc-finger motifs that mediate protein-protein interactions.
• The protein binds zinc ions via its LIM domains, which are essential for structural integrity and molecular recognition.
• CRIP2 is widely expressed in human tissues, with the most abundant expression observed in the heart, suggesting a role in cardiac biology.
• Alternative splicing of the CRIP2 gene yields multiple protein isoforms, potentially contributing to functional diversity.
• Post-translational modifications include acetylation and phosphorylation, as identified in proteomic studies.
• The three-dimensional structure of CRIP2 has been experimentally determined, providing insights into its molecular architecture.
• The canonical isoform has a predicted molecular weight of approximately 22.5 kDa, consistent with the 22 kDa band typically observed in western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CRIP2 protein (amino acids 1–208), which may generate polyclonal antibodies recognizing multiple epitopes; this can enhance sensitivity in denaturing western blot assays.
• Given the high sequence conservation across human, mouse, and rat, the antibody is expected to cross-react with these species; nevertheless, verify reactivity in your specific model system.
• For ELISA applications, consider using the recombinant immunogen as a positive control to establish standard curves and assay conditions.
• Optimize primary antibody dilution and incubation conditions empirically, as polyclonal responses can vary between production lots.
• Note that post-translational modifications (e.g., acetylation, phosphorylation) might influence antibody binding; if studying specific modified forms, confirm with appropriate peptide competition or mutant controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material procurement, bridging research and clinical assay development. This anti-CRIP2 polyclonal antibody supports cardiovascular biomarker research and assay prototyping. Beyond individual antibodies, we supply validated antibody pairs, bulk monoclonal/polyclonal antibodies, and tailored sourcing for rare targets, ensuring a seamless pipeline from concept to clinic.