Anti-CRIP1 Polyclonal Antibody for WB and ELISA - P50238

Core Product Specifications and Parameters

Biological Background: CRIP1 Function and Localization

• CRIP1 (Cysteine-rich protein 1), also known as CRHP (cysteine-rich heart protein), CRIP (cysteine-rich intestinal protein), and CRP1, is a small protein of 77 amino acids with a predicted molecular weight of ~8.5 kDa (9 kDa by gel analysis).
• It belongs to the LIM domain protein family, characterized by a cysteine-rich zinc-binding motif that facilitates protein-protein interactions and cellular signaling.
• CRIP1 is implicated in zinc ion homeostasis; it may function as an intracellular zinc transporter, facilitating absorption and cellular distribution of zinc.
• The protein undergoes post-translational modifications, including acetylation and methylation, which may regulate its activity and stability.
• No subcellular localization data is available in UniProt for CRIP1, but its role as a zinc transporter suggests a cytosolic or membrane-associated localization.
• The human CRIP1 gene (GeneID: 1396) encodes a single pass protein that is conserved across mammals, with cross-reactivity observed in mouse and rat.

Experimental Guidance and Technical Tips

• The antibody was produced using a recombinant fusion protein encompassing the full-length human CRIP1 sequence (amino acids 1-77, NP_001302.1). This design ensures recognition of the native protein’s complete structure.
• For Western blot (WB) analysis under reducing conditions, a single band at approximately 9 kDa is expected. Optimization of blocking and washing conditions may enhance signal specificity.
• In ELISA, the antibody can be used for direct or indirect detection; pairing with a recombinant CRIP1 protein or peptide may serve as a reliable positive control.
• Cross-reactivity is reported with human, mouse, and rat CRIP1, suggesting sequence conservation across these species. Nevertheless, it is advisable to verify compatibility in the specific experimental model.
• No recommended dilutions are provided; users should determine optimal antibody dilutions empirically based on their assay conditions and detection systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing high-quality antibodies like this anti-CRIP1 polyclonal antibody, along with a comprehensive portfolio of recombinant proteins, antibody pairs, and ancillary reagents. As a dedicated B2B sourcing partner, CamelBio assists customers in validating and scaling up raw materials for IVD assay development, covering targets involved in metal homeostasis and cellular signaling. For specialized needs, CamelBio can also facilitate the sourcing of rare-target raw materials and provide technical consulting to ensure seamless project advancement.