Anti-CRHR1 Rabbit Polyclonal Antibody for WB and ELISA - P34998

Core Product Specifications and Parameters

Biological Background: CRHR1 Function and Localization

• CRHR1 is a G protein-coupled receptor (GPCR) for corticotropin-releasing hormone (CRH) and urocortin (UCN), playing a central role in whole-body adaptation to stress.
  Related references:
  PMID:18292205
  PMID:18801728
  PMID:20966082

• Upon ligand binding, CRHR1 primarily couples to G(s) protein (GNAS) to stimulate adenylate cyclase and cAMP production, and can also signal via G(q) protein to generate DAG and IP3 second messengers.
  Related references:
  PMID:18292205
  PMID:32004469
  PMID:32004470
  PMID:14657255

• CRHR1-dependent signaling is a primary mediator of neuroendocrine, autonomic, and behavioral stress responses; it activates the hypothalamus-pituitary-adrenal (HPA) axis, leading to corticotropin (ACTH) secretion.

• In the brain, CRHR1 mediates both anxiogenic and anxiolytic effects in distinct neuronal populations and critically contributes to stress-induced drug seeking and addiction.

• Predominantly expressed in the cerebellum, pituitary gland, cerebral cortex, and olfactory lobe.

• Localized at the cell membrane, CRHR1 undergoes alternative splicing and is subject to glycosylation and phosphorylation.

• This receptor does not bind CRH with high affinity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 24-120) of human CRHR1, suitable for detecting total receptor levels in denaturing Western blot assays.
• For ELISA applications, consider using the antibody as a capture or detection reagent; validate specificity with recombinant CRHR1 protein and appropriate controls.
• Cross-reactivity with mouse and rat allows application in rodent stress models; always verify optimal antibody dilutions and sample preparation conditions for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, technical services, and consulting from concept to clinic. For CRHR1-related research and diagnostic development, we supply validated polyclonal antibodies, paired antibody sets for sandwich ELISA, and ancillary reagents. Contact us to discuss bulk procurement or custom sourcing of any rare-target raw materials.