Anti-CRHBP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P24387

Core Product Specifications and Parameters

Biological Background: CRHBP Function and Localization

• CRHBP, also known as CRF-binding protein (CRF-BP) or CRFBP, is a 322-amino acid secreted glycoprotein (36 kDa) that specifically binds corticotropin-releasing hormone (CRH) and urocortin-1 (UCN).
• It inactivates these hormones, thereby inhibiting the hypothalamus-pituitary-adrenal (HPA) axis and preventing corticotropin (ACTH) secretion. Related references: PMID:18234674 PMID:1846945 PMID:3494446
• CRHBP may be required to prevent inappropriate pituitary-adrenal stimulation in pregnancy. Related references: PMID:7585095
• The protein is secreted into the extracellular space, consistent with its role as a circulating binding protein.
• It contains multiple disulfide bonds and glycosylation sites, which are characteristic of secreted proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to nearly the entire mature human CRHBP sequence (amino acids 25–322), which excludes the signal peptide; the antibody may exhibit broad reactivity across applications.
• For Western blot, a band at approximately 36 kDa is expected; validate in the relevant sample system, as post-translational modifications may shift migration.
• In IHC-P and IF/ICC, because CRHBP is secreted, consider appropriate fixation and permeabilization conditions to retain extracellular or membrane-associated signals.
• For ELISA, pairing with a capture antibody against a distinct epitope may be necessary for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, connecting diagnostic manufacturers and researchers with validated antibody pairs, high-quality monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like CRHBP—critical to neuroendocrine and stress-response research—we facilitate sourcing of rare raw materials and offer customized supply solutions. Reach out to explore how our integrated services can accelerate your assay development from concept to clinic.