Anti-CRELD1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96HD1

Core Product Specifications and Parameters

Biological Background: CRELD1 Function and Localization

• CRELD1 is a protein disulfide isomerase and contains cysteine-rich EGF-like domains.
• It functions in promoting the localization of acetylcholine receptors (AChRs) to the plasma membrane.
• CRELD1 is a membrane protein, predicted to have a signal peptide and transmembrane helix.
• Highly expressed in fetal lung, liver, and kidney, and in adult heart, brain, and skeletal muscle, with weaker expression in placenta, fetal brain, and adult lung, liver, kidney, and pancreas.
• Post-translational modifications include glycosylation and disulfide bond formation; its redox-active center is characteristic of isomerases.
• Alternatively spliced isoforms may exist; mutations in CRELD1 are associated with diseases including atrioventricular septal defects, epilepsy, and intellectual disability.

Experimental Guidance and Technical Tips

• When using this polyclonal antibody, consider optimizing dilution for each application and validating in the relevant sample system.
• For western blotting, positive tissue lysates such as heart, brain, or skeletal muscle may yield a band around 45 kDa.
• For immunofluorescence/immunocytochemistry, ensure appropriate permeabilization and fixation methods to access the membrane-localized antigen.
• Use appropriate negative controls and known positive samples to confirm specificity across human, mouse, and rat species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw material procurement, providing a comprehensive range of antibodies and reagents to accelerate your diagnostic development. For researchers and manufacturers working on CRELD1 and related membrane proteins, CamelBio offers reliable access to polyclonal and monoclonal antibodies, validated pairs, and bulk ancillary supplies. Our one-stop platform streamlines sourcing from concept to clinic, ensuring you have the critical raw materials for successful assay development.