Anti-CREG1 Rabbit Polyclonal Antibody for WB, ELISA - O75629

Core Product Specifications and Parameters

Biological Background: CREG1 Function and Localization

• Protein CREG1 (Cellular repressor of E1A-stimulated genes 1) is a secreted glycoprotein involved in the regulation of cell growth and differentiation.
• It functions as a transcriptional repressor, antagonizing the activation and cellular transformation induced by the adenovirus E1A protein.
• The growth-regulatory activity of CREG1 is dependent on its interaction with the insulin-like growth factor 2 receptor (IGF2R).
• CREG1 is localized to the extracellular space, consistent with its role as a secreted signaling molecule.
• The protein undergoes glycosylation and contains a signal peptide for secretion, as indicated by its classification as a glycoprotein and signal peptide-containing protein.
• CREG1 has been characterized by direct protein sequencing and proteomics identification, confirming its sequence and presence in human proteome studies.
• A three-dimensional structure of CREG1 is available, providing insights into its functional domains.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 110–190 of human CREG1, targeting a region likely to be accessible in both native and denatured conditions.
• For Western blot, expect a band around 24 kDa; glycosylation may cause slight shifts. Validate in relevant cell lines (e.g., HeLa, A549) or tissues (e.g., liver, kidney) where CREG1 is expressed.
• Due to the polyclonal nature, lot-to-lot consistency should be monitored; consider including a positive control lysate with each experiment.
• In ELISA, the antibody can be used for detection of recombinant CREG1 or endogenous protein in cell culture supernatants or serum samples. Optimize coating and detection conditions empirically.
• Cross-reactivity with mouse and rat CREG1 broadens experimental utility; however, verify specificity in your model system using knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers the CREG1 Rabbit pAb as part of our comprehensive portfolio of IVD raw materials, supporting researchers and diagnostic manufacturers in cell growth regulation and cancer biology. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets such as CREG1—ensuring you have reliable access to critical reagents from concept to clinic. Partner with CamelBio to streamline your assay development and procurement with a single, trusted source.