Anti-CREBZF Rabbit Polyclonal Antibody for WB and ELISA - Q9NS37

Core Product Specifications and Parameters

Biological Background: CREBZF (CREB/ATF bZIP transcription factor) Function and Localization

• CREBZF, also known as ZF, SMILE, or host cell factor-binding transcription factor Zhangfei, is a CREB/ATF bZIP transcription factor encoded by the CREBZF gene in humans.
• It strongly activates transcription when bound to HCFC1 and suppresses HSV protein expression in infected cells via HCFC1.
• CREBZF also inhibits HCFC1-dependent transcriptional activation by CREB3 and reduces CREB3 protein levels, and can down-regulate expression of some cellular genes.
• Subcellularly, CREBZF localizes predominantly to the nucleus.
• In adults, it is highly expressed in heart, liver, and skeletal muscle, moderately in kidney and pancreas, and barely detectable in lung; in fetal tissues, it is most abundant in kidney.
• The protein is classified as a phosphoprotein and is involved in transcription regulation, as highlighted by keywords such as Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1–354, suggesting the antibody may recognize multiple epitopes along the full-length sequence.
• For Western blotting, validate the antibody using appropriate positive control lysates (e.g., from heart, liver, or skeletal muscle tissues) and consider both reducing and non-reducing conditions to confirm specificity at the expected molecular weight of ~37 kDa.
• When performing ELISA, use recombinant CREBZF protein as a standard and optimize coating and detection conditions; cross-reactivity with mouse and rat homologs indicates conserved epitopes, so verify performance in those species if applicable.
• As a polyclonal antibody, batch-to-batch variability may occur; include internal reference samples in each experiment to ensure consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For transcription factor targets like CREBZF, we offer polyclonal antibodies and can assist in sourcing rare or custom raw materials to support gene regulation studies and assay development. Our comprehensive supply chain and technical consultation streamline the journey from concept to clinic.